PrimePCR™ Probe Assay: ABCB4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ABCB4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ABCB4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049168
Assay Design:   exonic
Chromosome Location:   7:87031477-87031572question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000356878 ENST00000530339 ENST00000512229 ENST00000299194 ENST00000543818 ENST00000543086 ENST00000546231 ENST00000360905 ENST00000355446 ENST00000397203 ENST00000589028 ENST00000440025 ENST00000359206 ENST00000265723 ENST00000453593 ENST00000358400 ENST00000545634 ENST00000265724 ENST00000543898 ENST00000373264 ENST00000373265 ENST00000409832 ENST00000551509 ENST00000317586 ENST00000299213 ENST00000366795

Gene Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq Jul 2008]

Gene Symbol:   ABCB4
Gene Name:   ATP-binding cassette, sub-family B (MDR/TAP), member 4
Aliases:   ABC21, GBD1, MDR2, MDR2/3, MDR3, PFIC-3, PGY3
RefSeq:   NC_000007.13 NT_007933.15 NG_007118.1
Ensembl:   ENSG00000005471
Entrez:   5244
Chromosome Mapping:   7q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.210000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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