PrimePCR™ Probe Assay: HAMP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HAMP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HAMP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050350
Assay Design:   exonic
Chromosome Location:   19:35775894-35776012question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000598398 ENST00000222304

Gene Information

The product encoded by this gene is involved in the maintenance of iron homeostasis and it is necessary for the regulation of iron storage in macrophages and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20 22 and 25 amino acids and these active peptides are rich in cysteines which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B also known as juvenile hemochromatosis a disease caused by severe iron overload that results in cardiomyopathy cirrhosis and endocrine failure. [provided by RefSeq Jul 2008]

Gene Symbol:   HAMP
Gene Name:   hepcidin antimicrobial peptide
Aliases:   HEPC, HFE2B, LEAP1, PLTR
RefSeq:   NC_000019.9 NG_011563.1 NT_011109.16
Ensembl:   ENSG00000105697
Entrez:   57817
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.810000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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