PrimePCR™ Probe Assay: PKP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PKP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PKP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052452
Assay Design:   exonic
Chromosome Location:   2:159537773-159537902question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000389757 ENST00000389759

Gene Information

Armadillo-like proteins are characterized by a series of armadillo repeats first defined in the Drosophila 'armadillo' gene product that are typically 42 to 45 amino acids in length. These proteins can be divided into subfamilies based on their number of repeats their overall sequence similarity and the dispersion of the repeats throughout their sequences. Members of the p120(ctn)/plakophilin subfamily of Armadillo-like proteins including CTNND1 CTNND2 PKP1 PKP2 PKP4 and ARVCF. PKP4 may be a component of desmosomal plaque and other adhesion plaques and is thought to be involved in regulating junctional plaque organization and cadherin function. Multiple transcript variants have been found for this gene but the full-length nature of only two of them have been described so far. These two variants encode distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   PKP4
Gene Name:   plakophilin 4
Aliases:   FLJ31261, FLJ42243, p0071
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000144283
Entrez:   8502
Chromosome Mapping:   2q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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