PrimePCR™ Probe Assay: NPY1R, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NPY1R, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPY1R, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053441
Assay Design:   exonic
Chromosome Location:   4:164246799-164246909question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000296533 ENST00000509586 ENST00000504391 ENST00000512819

Gene Information

Neuropeptide Y (NPY; MIM 162640) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities including effects on psychomotor activity food intake regulation of central endocrine secretion and potent vasoactive effects on the cardiovascular system. Two major subtypes of NPY (Y1 and Y2) have been defined by pharmacologic criteria. NPY receptors such as NPY1R have been identified in a variety of tissues including brain spleen small intestine kidney testis placenta and aortic smooth muscle (Herzog et al. 1992 [PubMed 1321422]).[supplied by OMIM Mar 2008]

Gene Symbol:   NPY1R
Gene Name:   neuropeptide Y receptor Y1
Aliases:   NPYR
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164128
Entrez:   4886
Chromosome Mapping:   4q31.3-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.070000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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