PrimePCR™ Probe Assay: Hfe, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hfe, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hfe, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0045443
Assay Design:   Intron-spanning
Chromosome Location:   17:45521365-45523027question
Amplicon Length:   168
Splice Variants Targeted:   ENSRNOT00000010050 ENSRNOT00000002321 ENSRNOT00000022881 ENSRNOT00000022809 ENSRNOT00000022810 ENSRNOT00000016555 ENSRNOT00000005685 ENSRNOT00000016380 ENSRNOT00000064438 ENSRNOT00000026792 ENSRNOT00000050763

Gene Information

membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006]

Gene Symbol:   Hfe
Gene Name:   Hereditary hemochromatosis protein homolog
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000016967
Entrez:   29199
Chromosome Mapping:   17p11-q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 37.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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