PrimePCR™ Probe Assay: OXR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: OXR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OXR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041202
Assay Design:   exonic
Chromosome Location:   8:107763274-107763402question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000531443 ENST00000517566 ENST00000312046 ENST00000519415 ENST00000449762 ENST00000297447 ENST00000521592 ENST00000445937 ENST00000442977 ENST00000452423

Gene Information

Description Not Available

Gene Symbol:   OXR1
Gene Name:   oxidation resistance 1
Aliases:   FLJ10125, FLJ38829, FLJ40849, FLJ41673, FLJ42450, FLJ45656
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000164830
Entrez:   55074
Chromosome Mapping:   8q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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