PrimePCR™ Probe Assay: PTK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024199
Assay Design:   Exonic
Chromosome Location:   8:141727697-141727807question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000522684 ENST00000519654 ENST00000519465 ENST00000517887 ENST00000521059 ENST00000523539 ENST00000340930 ENST00000519419 ENST00000430260 ENST00000521986 ENST00000522424 ENST00000521562 ENST00000523388 ENST00000395214 ENST00000535192 ENST00000395218 ENST00000354438 ENST00000395221 ENST00000538769

Gene Information

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene but the full-length natures of only three of them have been determined. [provided by RefSeq Dec 2010]

Gene Symbol:   PTK2
Gene Name:   PTK2 protein tyrosine kinase 2
Aliases:   FADK, FAK, FAK1, FRNK, pp125FAK
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000169398
Entrez:   5747
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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