PrimePCR™ Probe Assay: SOCS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SOCS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SOCS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024407
Assay Design:   Exonic
Chromosome Location:   16:11349223-11349323question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000332029

Gene Information

This gene encodes a member of the STAT-induced STAT inhibitor (SSI) also known as suppressor of cytokine signaling (SOCS) family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines including IL2 IL3 erythropoietin (EPO) CSF2/GM-CSF and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action which is required for normal postnatal growth and survival. [provided by RefSeq Jul 2008]

Gene Symbol:   SOCS1
Gene Name:   suppressor of cytokine signaling 1
Aliases:   CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000185338
Entrez:   8651
Chromosome Mapping:   16p13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.730000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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