PrimePCR™ Probe Assay: MAPK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MAPK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAPK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050000
Assay Design:   exonic
Chromosome Location:   22:22142994-22153327question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000215832 ENST00000398822 ENST00000544786

Gene Information

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation differentiation transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation this kinase translocates to the nucleus of the stimulated cells where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein but differing in the UTRs have been reported for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPK1
Gene Name:   mitogen-activated protein kinase 1
Aliases:   ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
RefSeq:   NC_000022.10 NG_023054.1 NT_011520.12
Ensembl:   ENSG00000100030
Entrez:   5594
Chromosome Mapping:   22q11.2,22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.830000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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