PrimePCR™ Probe Assay: NGF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NGF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NGF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030939
Assay Design:   Intron-spanning
Chromosome Location:   1:115829356-115836288question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000369512

Gene Information

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5) and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq Jul 2008]

Gene Symbol:   NGF
Gene Name:   nerve growth factor (beta polypeptide)
Aliases:   Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
RefSeq:   NC_000001.10 NG_007944.1 NT_032977.9
Ensembl:   ENSG00000134259
Entrez:   4803
Chromosome Mapping:   1p13.1

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