PrimePCR™ Probe Assay: Numb, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Numb, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Numb, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029982
Assay Design:   Intron-spanning
Chromosome Location:   12:83808138-83811330question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000117217 ENSMUST00000129335 ENSMUST00000154043 ENSMUST00000021647 ENSMUST00000155112 ENSMUST00000135962

Gene Information

This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2013]

Gene Symbol:   Numb
Gene Name:   Numb gene homolog (Drosophila)
Aliases:   m-Nb, m-numb, mnb
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000021224
Entrez:   18222
Chromosome Mapping:   12|12 D3

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