PrimePCR™ Probe Assay: CACNA1C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CACNA1C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CACNA1C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052759
Assay Design:   exonic
Chromosome Location:   12:2690767-2690902question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000335762 ENST00000399655 ENST00000480911 ENST00000399595 ENST00000399644 ENST00000399638 ENST00000399597 ENST00000399621 ENST00000399637 ENST00000399591 ENST00000399641 ENST00000347598 ENST00000399606 ENST00000399601 ENST00000344100 ENST00000399629 ENST00000327702 ENST00000399649 ENST00000402845 ENST00000399603 ENST00000399634 ENST00000399617 ENST00000406454

Gene Information

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1 alpha-2/delta beta and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   CACNA1C
Gene Name:   calcium channel, voltage-dependent, L type, alpha 1C subunit
Aliases:   CACH2, CACN2, CACNL1A1, CCHL1A1, CaV1.2, MGC120730, TS
RefSeq:   NC_000012.11 NT_009759.16 NG_008801.1
Ensembl:   ENSG00000151067
Entrez:   775
Chromosome Mapping:   12p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.030000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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