PrimePCR™ Probe Assay: LCK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LCK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LCK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054815
Assay Design:   exonic
Chromosome Location:   1:32739941-32740035question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000336890 ENST00000482949 ENST00000495610 ENST00000461712 ENST00000373562 ENST00000477031 ENST00000373557 ENST00000333070 ENST00000373564

Gene Information

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation a PTK domain and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles and binds to cell surface receptors including CD4 and CD8 and other signaling molecules. Multiple alternatively spliced variants encoding the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   LCK
Gene Name:   lymphocyte-specific protein tyrosine kinase
Aliases:   LSK, YT16, p56lck, pp58lck
RefSeq:   NC_000001.10 NT_032977.9 NG_023387.1
Ensembl:   ENSG00000182866
Entrez:   3932
Chromosome Mapping:   1p34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.210000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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