PrimePCR™ Probe Assay: SNCAIP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SNCAIP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNCAIP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027677
Assay Design:   Intron-spanning
Chromosome Location:   5:121785558-121786317question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000509154 ENST00000261368 ENST00000261367 ENST00000379533 ENST00000379536 ENST00000503116 ENST00000504884 ENST00000414317 ENST00000447854 ENST00000379538 ENST00000542191

Gene Information

This gene encodes a protein containing several protein-protein interaction domains including ankyrin-like repeats a coiled-coil domain and an ATP/GTP-binding motif. The encoded protein interacts with alpha-synuclein in neuronal tissue and may play a role in the formation of cytoplasmic inclusions and neurodegeneration. A mutation in this gene has been associated with Parkinson's disease. Alternatively spliced transcript variants encoding different isoforms of this gene have been described but the full-length nature of only two have been determined. [provided by RefSeq Jul 2011]

Gene Symbol:   SNCAIP
Gene Name:   synuclein, alpha interacting protein
Aliases:   MGC39814, SYPH1, Sph1
RefSeq:   NC_000005.9 NG_011486.1 NT_034772.6
Ensembl:   ENSG00000064692
Entrez:   9627
Chromosome Mapping:   5q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.710000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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