PrimePCR™ Probe Assay: CDC42, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDC42, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDC42, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040659
Assay Design:   Intron-spanning
Chromosome Location:   1:22408235-22413224question
Amplicon Length:   196
Splice Variants Targeted:   ENST00000421089 ENST00000400259 ENST00000411827 ENST00000315554 ENST00000344548

Gene Information

The protein encoded by this gene is a small GTPase of the Rho-subfamily which regulates signaling pathways that control diverse cellular functions including cell morphology migration endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42 and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP) which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC42
Gene Name:   cell division cycle 42 (GTP binding protein, 25kDa)
Aliases:   CDC42Hs, G25K
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000070831
Entrez:   998
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.600000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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