PrimePCR™ Probe Assay: CTNNB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CTNNB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTNNB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053779
Assay Design:   exonic
Chromosome Location:   3:41266084-41266226question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000426215 ENST00000405570 ENST00000431914 ENST00000396183 ENST00000349496 ENST00000453024 ENST00000396185 ENST00000450969 ENST00000441708

Gene Information

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally this protein binds to the product of the APC gene which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC) pilomatrixoma (PTR) medulloblastoma (MDB) and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   CTNNB1
Gene Name:   catenin (cadherin-associated protein), beta 1, 88kDa
Aliases:   CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
RefSeq:   NC_000003.11 NT_022517.18 NG_013302.1
Ensembl:   ENSG00000168036
Entrez:   1499
Chromosome Mapping:   3p21

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