PrimePCR™ Probe Assay: EIF3D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF3D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF3D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032862
Assay Design:   Intron-spanning
Chromosome Location:   22:36919942-36920692question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000216190 ENST00000405442 ENST00000455547 ENST00000457241 ENST00000432675 ENST00000397177

Gene Information

Eukaryotic translation initiation factor-3 (eIF3) the largest of the eIFs is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq Jul 2008]

Gene Symbol:   EIF3D
Gene Name:   eukaryotic translation initiation factor 3, subunit D
Aliases:   EIF3S7, MGC126526, MGC17258, eIF3-p66, eIF3-zeta
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100353
Entrez:   8664
Chromosome Mapping:   22q13.1

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