PrimePCR™ Probe Assay: RAC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024025
Assay Design:   Exonic
Chromosome Location:   7:6443404-6443512question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000348035

Gene Information

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events including the control of cell growth cytoskeletal reorganization and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   RAC1
Gene Name:   ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Aliases:   MGC111543, TC-25, p21-Rac1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000136238
Entrez:   5879
Chromosome Mapping:   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 34.940000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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