PrimePCR™ Probe Assay: EIF2S2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EIF2S2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF2S2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024621
Assay Design:   Exonic
Chromosome Location:   20:32676667-32676820question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000374980

Gene Information

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits alpha beta and gamma with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq Jul 2008]

Gene Symbol:   EIF2S2
Gene Name:   eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
Aliases:   DKFZp686L18198, EIF2, EIF2B, EIF2beta, MGC8508
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000125977
Entrez:   8894
Chromosome Mapping:   20pter-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.570000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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