PrimePCR™ Probe Assay: DAXX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DAXX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DAXX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040008
Assay Design:   exonic
Chromosome Location:   6:33289222-33289514question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000455860 ENST00000436311 ENST00000440500 ENST00000419855 ENST00000399344 ENST00000383194 ENST00000429531 ENST00000438332 ENST00000266000 ENST00000374542 ENST00000446403 ENST00000453407 ENST00000414083 ENST00000399060 ENST00000383062 ENST00000428268 ENST00000414272 ENST00000453931 ENST00000454197 ENST00000433482 ENST00000445009

Gene Information

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins such as apoptosis antigen Fas centromere protein C and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications including sumoylation phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2008]

Gene Symbol:   DAXX
Gene Name:   death-domain associated protein
Aliases:   BING2, DAP6, EAP1, MGC126245, MGC126246
RefSeq:   NC_000006.11 NG_009876.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204209
Entrez:   1616
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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