PrimePCR™ Probe Assay: POU5F1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POU5F1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POU5F1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041056
Assay Design:   exonic
Chromosome Location:   6:31132365-31132494question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000340394 ENST00000325215 ENST00000395547 ENST00000336783 ENST00000382686 ENST00000564304 ENST00000570200 ENST00000566946 ENST00000440692 ENST00000340912 ENST00000544171 ENST00000327606 ENST00000259915 ENST00000512818 ENST00000441888 ENST00000471529 ENST00000454714 ENST00000433348 ENST00000549294 ENST00000437747 ENST00000429314 ENST00000548685 ENST00000544745 ENST00000308736 ENST00000465342 ENST00000391675 ENST00000433063 ENST00000419095 ENST00000548682 ENST00000412166 ENST00000434616 ENST

Gene Information

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development especially during early embryogenesis and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene t(6;22)(p21;q12) has been linked to tumor formation. Alternative splicing as well as usage of alternative translation initiation codons results in multiple isoforms one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1 3 8 10 and 12. [provided by RefSeq Mar 2010]

Gene Symbol:   POU5F1
Gene Name:   POU class 5 homeobox 1
Aliases:   MGC22487, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204531
Entrez:   5460
Chromosome Mapping:   6p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.050000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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