PrimePCR™ Probe Assay: ESR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ESR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ESR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049903
Assay Design:   exonic
Chromosome Location:   6:152332828-152382130question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000338799 ENST00000206249 ENST00000427531 ENST00000415488 ENST00000440973 ENST00000456483 ENST00000443427 ENST00000544394

Gene Information

This gene encodes an estrogen receptor a ligand-activated transcription factor composed of several domains important for hormone binding DNA binding and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer endometrial cancer and osteoporosis. Alternative splicing results in several transcript variants which differ in their 5' UTRs and use different promoters. [provided by RefSeq Jul 2008]

Gene Symbol:   ESR1
Gene Name:   estrogen receptor 1
Aliases:   DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
RefSeq:   NC_000006.11 NT_025741.15 NG_008493.1
Ensembl:   ENSG00000091831
Entrez:   2099
Chromosome Mapping:   6q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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