PrimePCR™ Probe Assay: DKK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DKK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DKK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050470
Assay Design:   exonic
Chromosome Location:   10:54076610-54076735question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000373970

Gene Information

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq Jul 2008]

Gene Symbol:   DKK1
Gene Name:   dickkopf homolog 1 (Xenopus laevis)
Aliases:   DKK-1, SK
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107984
Entrez:   22943
Chromosome Mapping:   10q11.2

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