PrimePCR™ Probe Assay: NCL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050834
Assay Design:   exonic
Chromosome Location:   2:232326541-232327912question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000322723 ENST00000454824 ENST00000417652 ENST00000453992 ENST00000436894

Gene Information

Nucleolin (NCL) a eukaryotic nucleolar phosphoprotein is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA termed U20. [provided by RefSeq Jul 2008]

Gene Symbol:   NCL
Gene Name:   nucleolin
Aliases:   C23, FLJ45706, FLJ59041
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115053
Entrez:   4691
Chromosome Mapping:   2q37.1

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