PrimePCR™ Probe Assay: LBP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LBP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051549
Assay Design:   exonic
Chromosome Location:   20:36993309-36995425question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000217407

Gene Information

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid lipopolysaccharide (LPS) on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI) the encoded protein binds LPS and interacts with the CD14 receptor probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins including BPI plasma cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP). Finally this gene is found on chromosome 20 immediately downstream of the BPI gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LBP
Gene Name:   lipopolysaccharide binding protein
Aliases:   MGC22233
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000129988
Entrez:   3929
Chromosome Mapping:   20q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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