PrimePCR™ Probe Assay: MAP4K4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP4K4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP4K4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027019
Assay Design:   Intron-spanning
Chromosome Location:   2:102490609-102493530question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000425019 ENST00000413150 ENST00000456652 ENST00000347699 ENST00000417294 ENST00000421882 ENST00000324219 ENST00000350198 ENST00000302217 ENST00000350878

Gene Information

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1 MAP2K4/MKK4 and MAP2K7/MKK7 which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP4K4
Gene Name:   mitogen-activated protein kinase kinase kinase kinase 4
Aliases:   FLH21957, FLJ10410, FLJ20373, FLJ90111, HGK, KIAA0687, NIK
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000071054
Entrez:   9448
Chromosome Mapping:   2q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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