PrimePCR™ Probe Assay: NMNAT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NMNAT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NMNAT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028299
Assay Design:   Intron-spanning
Chromosome Location:   1:183247720-183253853question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000287713 ENST00000294868

Gene Information

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member which is localized to the nucleus and is ubiquitously expressed; this enzyme is cytoplasmic and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NMNAT2
Gene Name:   nicotinamide nucleotide adenylyltransferase 2
Aliases:   C1orf15, KIAA0479, MGC2756, PNAT2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000157064
Entrez:   23057
Chromosome Mapping:   1q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 33.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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