PrimePCR™ Probe Assay: ACACB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACACB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACACB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029534
Assay Design:   Intron-spanning
Chromosome Location:   12:109675127-109677693question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000338432 ENST00000377848 ENST00000377854 ENST00000390027 ENST00000543201

Gene Information

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   ACACB
Gene Name:   acetyl-CoA carboxylase beta
Aliases:   ACC2, ACCB, HACC275
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000076555
Entrez:   32
Chromosome Mapping:   12q24.11

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