PrimePCR™ Probe Assay: Pde4a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Pde4a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Pde4a, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047968
Assay Design:   Exonic
Chromosome Location:   8:22288702-22288840question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000028272 ENSRNOT00000061100 ENSRNOT00000061099 ENSRNOT00000074313 ENSRNOT00000041381 ENSRNOT00000012792 ENSRNOT00000029340

Gene Information

human homolog catalyzes the hydrolysis of cAMP; activity is inhibited by rolipram [RGD, Feb 2006]

Gene Symbol:   Pde4a
Gene Name:   CAMP-specific 3',5'-cyclic phosphodiesterase 4A
Aliases:   Not Available
RefSeq:   NM_013101
Ensembl:   ENSRNOG00000020828
Entrez:   25638
Chromosome Mapping:   8q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.110000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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