PrimePCR™ Variant Mutation Detection Assay: ORF3a S171L, SARS-CoV-2

PrimePCR

The PrimePCR SARS-CoV-2 Single Mutation Assay are single-tube mutation detection assays designed to detect specific mutations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome. Assays contain unlabeled PCR primers and different dye-labeled, allele-specific probes plus a primer and probe combination for internal positive control.

Info:   Assay differentiates between reference and S171L allele and includes an internal amplification control primer/probe assay.

 
Fluorophore:   FAM,HEX,CY5
List Price:    $916.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Variant Mutation Detection
Unique Assay ID:   qSC2Mut0020215
Amplicon Length:   91
Nucleotide Mutation Detection:   25904C>T
Amino Acid Change:   S171L
MIQE Context :question   ACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTT[C/T]AGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAA

Gene Information

ORF3a is a 827 nucleotide sequence that encodes an accessory protein located on the cell surface. This region is involved in pro-inflammatory cytokine and chemokine production through its activation of various signal transduction pathways, including C-Jun N-terminal kinase (JNK) and nuclear factor kapa light-chain-enhancer of activated B cells (NF-KB).

Gene Symbol:   ORF3a
Gene Name:   ORF3a
RefSeq:   NC_045512.2
Ensembl:   ENSSASG00005000006
Entrez:   43740569

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