PrimePCR™ Multiple Mutation Detection Assay: S 477N, 478K, 493R, 496S, 498R, SARS-CoV-2

PrimePCR

The PrimePCR SARS-CoV-2 Multiple Mutation RT-PCR Assay* is a multiplexed panel designed to detect and differentiate in a single well four key mutations identified in the severe respiratory syndrome coronavirus 2 (SARS-CoV-2) genome.

Info:   Assay differentiates between Delta, Omicron BA.1 and Omicron BA.2 SARS-CoV-2 variants and includes an internal amplification control primer/probe assay.

 
Fluorophore:   FAM, HEX, Texas Red, ATTO647, Cy5.5
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Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Variant Mutation Detection
Unique Assay ID:   qSC2Mut0020236
Amplicon Length:   336
Nucleotide Mutation Detection:   23040A>G, 23055A>G,22992G>A, 22995C>A,23040A>G, 23048G>A, 23055A>G,22995C>A
Amino Acid Change:   477N, 478K, 493R, 496S, 498R
MIQE Context :question   AAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTA[G/A]CA[C/A]ACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTAC[A/G]ATCATAT[G/A]GTTTCC[A/G]ACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAA

Gene Information

The S gene is a 3822 nucleotide sequence that encodes a glycoprotein responsible for the invasion of host cells. This spike protein consisted of two subunits: S1 contains a receptor-binding domain that binds to ACE2 on the host cell membrane, and S2 mediates fusion between the cell membranes of the virus and the cell. Mutations in either domain of the spike protein may impact the virus’ transmissibility or infectivity.

Gene Symbol:   S
Gene Name:   Surface Glycoprotein
Aliases:   GU280_gp02, spike glycoprotein
RefSeq:   NC_045512.2
Ensembl:   ENSSASG00005000004
Entrez:   43740568

Type :   gBlock Gene Fragments
For instructions on preparing the positive controls, please see the assay instruction manual.
Sequence :  

Click Here to select the positive control sequence. Then to copy it to your clipboard, press Ctrl + C for Windows users or ⌘ + C for Mac users.
   
Copy the above positive control sequence and go to the link below and paste to order.
Order from IDT:   https://www.idtdna.com/site/Order/gblockentry
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