PrimePCR™ Probe Assay: AGO1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AGO1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AGO1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023588
Assay Design:   Exonic
Chromosome Location:   1:17891888-17892522question
Amplicon Length:   78
Splice Variants Targeted:   AT1G48410.1 AT1G48410.2 AT1G48410.3 AT1G56440.1 AT1G56440.2

Gene Information

Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.

Gene Symbol:   AGO1
Gene Name:   Stabilizer of iron transporter SufD / Polynucleotidyl transferase
Aliases:   Not Available
RefSeq:   NM_001198240 NM_103737 NM_179453
Ensembl:   AT1G48410
Entrez:   841262
Chromosome Mapping:   Not Available

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