PrimePCR™ Probe Assay: MSL3, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MSL3, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MSL3, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023791
Assay Design:   Exonic
Chromosome Location:   1:21548329-21548436question
Amplicon Length:   78
Splice Variants Targeted:   AT4G20040.1 AT1G58200.1 AT1G58200.2

Gene Information

A member of MscS-like gene family, structurally very similar to MSL2, comprising of an N-terminal chloroplast transit peptide, five trans-membrane helices and a C-terminal cytoplasmic domain. Mutant plants showed abnormalities in the size and shape of plastids. MSL3-GFP was localized to discrete foci on the plastid envelope and co-localize with the plastid division protein AtMinE. MSL3 was capable of increasing the osmotic-shock survival of a mutant bacterial strain lacking MS-ion-channel activity.

Gene Symbol:   MSL3
Gene Name:   MSCS-like 3
Aliases:   Not Available
RefSeq:   NM_202317 NM_104601
Ensembl:   AT1G58200
Entrez:   842187
Chromosome Mapping:   Not Available

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