This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: COL1A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL1A1, Human
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone cornea dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV Ehlers-Danlos syndrome type VIIA Ehlers-Danlos syndrome Classical type Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22 where this gene and the gene for platelet-derived growth factor beta are located are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of the growth factor. Two transcripts resulting from the use of alternate polyadenylation signals have been identified for this gene. [provided by R. Dalgleish Feb 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.