PrimePCR™ SYBR® Green Assay: PLOD2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0052848

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLOD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLOD2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045587
Assay Design:   exonic
Chromosome Location:   3:145788367-145788481question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000461497 ENST00000282903 ENST00000360060 ENST00000494950

Gene Information

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   PLOD2
Gene Name:   procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Aliases:   LH2, TLH
RefSeq:   NC_000003.11 NG_009251.1 NT_005612.16
Ensembl:   ENSG00000152952
Entrez:   5352
Chromosome Mapping:   3q24

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