PrimePCR™ Probe Assay: GPR133, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GPR133, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPR133, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023959
Assay Design:   Exonic
Chromosome Location:   12:131616270-131620598question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000261654 ENST00000535015 ENST00000376682 ENST00000335486 ENST00000543617

Gene Information

The adhesion G-protein-coupled receptors (GPCRs) including GPR133 are membrane-bound proteins with long N termini containing multiple domains. GPCRs or GPRs contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al. 2004 [PubMed 15203201]).[supplied by OMIM Nov 2010]

Gene Symbol:   GPR133
Gene Name:   G protein-coupled receptor 133
Aliases:   DKFZp434B1272, FLJ16770, MGC138512, MGC138514, PGR25
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000111452
Entrez:   283383
Chromosome Mapping:   12q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.730000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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