PrimePCR™ Probe Assay: PCDH20, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCDH20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCDH20, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024067
Assay Design:   Exonic
Chromosome Location:   13:61987589-61987727question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000409204 ENST00000409186 ENST00000358674

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH20
Gene Name:   protocadherin 20
Aliases:   FLJ22218, PCDH13
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000197991
Entrez:   64881
Chromosome Mapping:   13q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.890000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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