PrimePCR™ Probe Assay: PLEKHB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: PLEKHB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLEKHB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024098
Assay Design:   Exonic
Chromosome Location:   11:73361648-73361747question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000354190 ENST00000398492 ENST00000536527 ENST00000227214 ENST00000398494 ENST00000545798 ENST00000539157 ENST00000546251 ENST00000535582 ENST00000538227 ENST00000539549 ENST00000543524 ENST00000541597 ENST00000535129 ENST00000542389 ENST00000540431 ENST00000542185

Gene Information

Description Not Available

Gene Symbol:   PLEKHB1
Gene Name:   pleckstrin homology domain containing, family B (evectins) member 1
Aliases:   KPL1, PHR1, PHRET1
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000021300
Entrez:   58473
Chromosome Mapping:   11q13.5-q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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