PrimePCR™ Probe Assay: LUC7L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LUC7L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: LUC7L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024181
Assay Design:   Exonic
Chromosome Location:   16:249061-249213question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000337351 ENST00000293872 ENST00000397783 ENST00000397780 ENST00000430864

Gene Information

The LUC7L gene may represent a mammalian heterochromatic gene encoding a putative RNA-binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex that is normally required for 5-prime splice site selection (Tufarelli et al. 2001 [PubMed 11170747]).[supplied by OMIM Mar 2008]

Gene Symbol:   LUC7L
Gene Name:   LUC7-like (S. cerevisiae)
Aliases:   FLJ10231, LUC7-LIKE, LUC7B1, Luc7, SR+89, hLuc7B1
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000007392
Entrez:   55692
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.510000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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