PrimePCR™ Probe Assay: PRKAG2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRKAG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRKAG2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024445
Assay Design:   Exonic
Chromosome Location:   7:151262806-151262945question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000418337 ENST00000287878 ENST00000492843 ENST00000392801 ENST00000433631

Gene Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit a noncatalytic beta subunit and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome) progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKAG2
Gene Name:   protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Aliases:   AAKG, AAKG2, CMH6, H91620p, WPWS
RefSeq:   NC_000007.13 NT_007914.15 NG_007486.1
Ensembl:   ENSG00000106617
Entrez:   51422
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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