PrimePCR™ Probe Assay: SOCS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SOCS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SOCS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024741
Assay Design:   Exonic
Chromosome Location:   17:76354029-76354232question
Amplicon Length:   174
Splice Variants Targeted:   ENST00000330871

Gene Information

This gene encodes a member of the STAT-induced STAT inhibitor (SSI) also known as suppressor of cytokine signaling (SOCS) family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines including IL6 IL10 and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis and placental development. [provided by RefSeq Jul 2008]

Gene Symbol:   SOCS3
Gene Name:   suppressor of cytokine signaling 3
Aliases:   ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3
RefSeq:   NC_000017.10 NG_016851.1 NT_010783.15
Ensembl:   ENSG00000184557
Entrez:   9021
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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