PrimePCR™ Probe Assay: ZNF498, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF498, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF498, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024885
Assay Design:   Exonic
Chromosome Location:   7:99229332-99229491question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000394152 ENST00000334715 ENST00000262941

Gene Information

This gene encodes a protein that bears some similarity to zinc finger proteins which are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants have been identified but the full-length nature for most of them has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF498
Gene Name:   zinc finger protein 498
Aliases:   ZSCAN25
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000197037
Entrez:   221785
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.110000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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