PrimePCR™ Probe Assay: ATP5A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP5A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP5A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025064
Assay Design:   Exonic
Chromosome Location:   18:43671666-43671799question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000398752 ENST00000282050 ENST00000542290

Gene Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled with a stoichiometry of 3 alpha 3 beta and a single representative of the other 3. The proton channel consists of three main subunits (a b c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9 2 and 16. [provided by RefSeq Mar 2012]

Gene Symbol:   ATP5A1
Gene Name:   ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Aliases:   ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000152234
Entrez:   498
Chromosome Mapping:   18q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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