PrimePCR™ Probe Assay: NCALD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCALD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCALD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025383
Assay Design:   Exonic
Chromosome Location:   8:102700314-102700481question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000220931 ENST00000521599 ENST00000519508 ENST00000395923 ENST00000311028

Gene Information

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   NCALD
Gene Name:   neurocalcin delta
Aliases:   MGC33870, MGC74858
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000104490
Entrez:   83988
Chromosome Mapping:   8q22.2

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