PrimePCR™ Probe Assay: PSMB11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSMB11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSMB11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032081
Assay Design:   Exonic
Chromosome Location:   14:23512521-23512611question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000408907

Gene Information

Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7 beta-1 to -7 beta-1 to -7 and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307) beta-2 (PSMB7; MIM 604030) and beta-5 (PSMB5; MIM 600306). Three additional subunits beta-1i (PSMB9; MIM 177045) beta-2i (PSMB10; MIM 176847) and beta-5i (PSMB8; MIM 177046) are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11 or beta-5t is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al. 2007 [PubMed 17540904]).[supplied by OMIM Mar 2008]

Gene Symbol:   PSMB11
Gene Name:   proteasome (prosome, macropain) subunit, beta type, 11
Aliases:   BETA5T, FLJ16369
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000222028
Entrez:   122706
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.250000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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