PrimePCR™ Probe Assay: CLDN3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLDN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLDN3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032466
Assay Design:   Exonic
Chromosome Location:   7:73184165-73184290question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000395145

Gene Information

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene a member of the claudin family is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq Jul 2008]

Gene Symbol:   CLDN3
Gene Name:   claudin 3
Aliases:   C7orf1, CPE-R2, CPETR2, HRVP1, RVP1
RefSeq:   NC_000007.13 NG_012023.1 NT_007933.15
Ensembl:   ENSG00000165215
Entrez:   1365
Chromosome Mapping:   7q11.23

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