PrimePCR™ Probe Assay: PSMB10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSMB10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSMB10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032554
Assay Design:   Exonic
Chromosome Location:   16:67970119-67970613question
Amplicon Length:   171
Splice Variants Targeted:   ENST00000358514

Gene Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family also known as the T1B family that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq Jul 2008]

Gene Symbol:   PSMB10
Gene Name:   proteasome (prosome, macropain) subunit, beta type, 10
Aliases:   FLJ00366, LMP10, MECL1, MGC1665, beta2i
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000205220
Entrez:   5699
Chromosome Mapping:   16q22.1

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