PrimePCR™ Probe Assay: ALX4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ALX4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALX4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039036
Assay Design:   exonic
Chromosome Location:   11:44284287-44284435question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000329255

Gene Information

This gene encodes a paired-like homeodomain transcription factor expressed in the mesenchyme of developing bones limbs hair teeth and mammary tissue. Mutations in this gene cause parietal foramina 2 (PFM2); an autosomal dominant disease characterized by deficient ossification of the parietal bones. Mutations in this gene also cause a form of frontonasal dysplasia with alopecia and hypogonadism; suggesting a role for this gene in craniofacial development mesenchymal-epithelial communication and hair follicle development. Deletion of a segment of chromosome 11 containing this gene del(11)(p11p12) causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies mental retardation multiple exostoses and genital abnormalities in males. In mouse this gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq Oct 2009]

Gene Symbol:   ALX4
Gene Name:   ALX homeobox 4
Aliases:   FND2, KIAA1788
RefSeq:   NC_000011.9 NG_015809.1 NT_009237.18
Ensembl:   ENSG00000052850
Entrez:   60529
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.840000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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