PrimePCR™ Probe Assay: HMMR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HMMR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HMMR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039054
Assay Design:   exonic
Chromosome Location:   5:162896647-162896759question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000416990 ENST00000520345 ENST00000522094 ENST00000353866 ENST00000393915 ENST00000432118 ENST00000358715

Gene Information

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins it forms a complex with BRCA1 and BRCA2 thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   HMMR
Gene Name:   hyaluronan-mediated motility receptor (RHAMM)
Aliases:   CD168, IHABP, MGC119494, MGC119495, RHAMM
RefSeq:   NC_000005.9 NG_023309.1 NT_023133.13
Ensembl:   ENSG00000072571
Entrez:   3161
Chromosome Mapping:   5q33.2-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.740000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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