PrimePCR™ Probe Assay: WFDC2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: WFDC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WFDC2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039098
Assay Design:   exonic
Chromosome Location:   20:44110086-35660648question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000372676 ENST00000339946 ENST00000342873

Gene Information

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain or WAP Signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein which may be involved in sperm maturation. [provided by RefSeq Jul 2008]

Gene Symbol:   WFDC2
Gene Name:   WAP four-disulfide core domain 2
Aliases:   EDDM4, HE4, MGC57529, WAP5, dJ461P17.6
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101443
Entrez:   10406
Chromosome Mapping:   20q12-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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